However, the roles of ER stress and autophagy in blood-spinal cord barrier disruption have not been discussed in acute spinal cord trauma. In order to carry out comparative studies between animal models and humans, a precise phenotypic characterization should be performed, accounting for both muscle development and metabolism (Capitanio et al., 2005(Capitanio et al., , 2009, ... Ectopic secretion of lysosomal proteases can, in fact, degradate extracellular matrix, contributing to atherosclerosis, arthritis, aneurysms formation, and other diseases [63]. PDF | Obesity is one of the risk factors associated with cardiovascular diseases, hypertension, abnormal liver function, diabetes, and cancers. 0000003517 00000 n In rat hearts, 6 stress response and 4 other proteins were identified: Gstp1, glutathione S-transferase P; Hspa8, heat shock cognate 71kDa protein; Mpst, 3-mercaptopyruvate sulfurtransferase; Pdia3, protein disulfide-isomerase A3; Prdx2, peroxiredoxin-2; Rnh1, ribonuclease inhibitor; Anxa5, annexin A5; Serpina1, alpha-1-antiproteinase (2 proteoforms); Serpina3k, serine protease inhibitor A3K; Serpina3l, serine protease inhibitor A3L. Statistical analysis was performed by Student’s T-Test (n=3; * p<0.05). 2010), yet experience suggests that this strain has a higher background incidence of PCM in young animals compared to other strains. Wistar rats are more active than others like Sprague Dawley rats. 0000007736 00000 n Fasting was used prior to all assays because vehicle, standard drugs, and samples were administered orally (by gavage). The results revealed that IL‑1β in the serum and IALF decreased in the OAE group, whereas IL‑4 increased, and histological evaluation indicated that the OAE group had a clear treatment response. Furthermore, autophagy is retained in triceps whereas an enhanced fusion, decrement of mitophagy and of regenerative potential is observed in aged gastrocnemius muscle. DOI: 10.1007/s00280-017-3364-z. The aim was to detect the anti-neuroinflammation effect of ASC transplantation and, Increased levels of reactive oxygen species (ROS) cause oxidative stress and are believed to play a key role in the development of age-related diseases and mammalian aging in general by oxidizing proteins, lipids, and DNA. Plasma was immediately decanted after centrifugation and stored at −25°C until analyzed. Hence, this study aimed to investigate the anti-obesity activity of Elateriospermum tapos (E. tapos) shell extract in obesity induced Sprague Dawley rats. The underlying oxidative modifications were also studied at the molecular level. The proposed model has been validated through an extensive literature analysis, which confirmed that 94% of the identified genes and miRs are related with cardiac senescence. Suppression of insulin/IGF-1 signaling prevents cardiac aging associated with improved protein homeostasis in the heart. This work presents an experimental study with the intervention of GM rice feeding by Sprague Dawley rats. 0000001122 00000 n Aging is a natural, multifactorial and multiorganic phenomenon wherein there are gradual physiological and pathological changes over time. Proteins significantly altered (ANOVA and Tukey, p < 0.01) are expressed as a ratio of spot volume variations in old adult and senescent rats versus young (6 months) control, respectively. 0000005439 00000 n We observed that fibroblasts from LLI displayed significantly lower complex I-driven ATP synthesis and higher production of H2O2 in comparison with old subjects. For proteins having proteoforms with opposite trends in the same group, the global protein expression is reported in frames. Despite a lack of left ventricular hypertrophy, MyBP-C(+/-) hearts exhibited elevated end-diastolic pressure and decreased dP/dtmax, which was normalized following dobutamine infusion. – Uchino E., Tsuzuki, T. & Inoue, K. The effects of age and sex on seven elements in Sprague-Dawley rat organs. injection of pentylenetetrazol (PTZ, 70–80 mg kg −1); some pups of each litter were used as controls. Literature data show beneficial effects of exercise on the impairment of mitochondrial biogenesis and dynamics, and on the decrease in the mitophagic capacity associated to aging. Trueness based on bias and the percentage of recovery showed bias … The SRG rats have a smaller spleen compared to wild-type Sprague Dawley rats and are essentially athymic (Fig 1J–1L), resulting in low viable cell yield from the thymus. Conclusion: However, the expression of type II collagen in the articular cartilage increased in the OAE group as compared with the OA group, whereas ADAMTS5 expression decreased. We randomized 24 rats into 4 groups as followings: Group A and B, received saline injections and autologous transplantation of ASCs 4 weeks after sham burn, respectively; Group C and D, received saline injections and autologous transplantation 4 weeks after burn injuries. This study highlights the importance and impact of ageing and anti-cancer therapy-induced cardiotoxicity. Relative to the, The aim of the present study was to investigate potential alterations in the articular cartilage in a rat model of monosodium iodoacetate (MIA)‑induced osteoarthritis (OA) with or without treatment with moderate treadmill exercise. Sprague Dawley rats in health. 2. Protein kinase A treatment abolished basal differences in rates of cross-bridge recruitment between MyBP-C(+/-) and wild-type myocardium. Heart failure is a typical age-associated disease. Liver LC3B1, LC3B2, Beclin-1, caspase 9 protein expression was analyzed by western blotting. B) Validation of Hspa8 protein by immunoblotting in young, old adult and senescent rat cardiac muscles. Two groups of Sprague Dawley and 2 groups of Lister-Hooded rats were controls for the gavage and diet administration. The STZ-induced group received 35 mg / kg STZ by intraperitoneal injection while the STZ and HFD-induced group received the STZ after 14 days high fat diet treatment. Thus, ASC transplantation post-burn scars shows the potential promising clinical benefits. The Sprague Dawley rat and Long–Evans rat were developed from Wistar rats. A total of 30 male Sprague‑Dawley rats were randomly divided into three groups (n=10), including the control, OA and OA with treadmill exercise (OAE) groups. a role, as Sprague-Dawley (SD) and Wistar rats on a restricted diet havea lowerincidenceof PCM(Kemi et al.2000).SD rats have been widely used for toxicology studies (Keenan et al. Alterations in articular cartilage and synovium were also evaluated by histology, immunohistochemistry, western blotting and reverse transcription‑quantitative polymerase chain reaction. High levels of dihydrolipoyllysine-residue acetyltransferase (Dlat) and ATP synthase subunit alpha (Atp5a1) are detected in triceps and gastrocnemius, respectively. Proteins significantly altered (ANOVA and Tukey, p<0.01) are expressed as a ratio of spot volume variations in old adult and senescent rats versus young (6 months) control, respectively. 0000012654 00000 n gestational day (GD) 6 of their mothers. In the present study, we respectively detected the roles of ER stress and. Why are you such a great model? 360 Sprague-Dawley rats (179 males and 181 females) and a 26% incidence was seen in 254 Swiss mice (101 males and 153 females) used as untreated control animals in an 18-month series of carcinogenesis experiments. (V/m)a I 0 M 412 F 405 M+F 817 II 5 M 401 F 410 M+F 811 III 25 M 209 F 202 M+F 411 IV 50 M 207 F 202 M+F 409 Total 2448 a Treatment with GSM-RFR 1.8GHz for 19h/day started on the 12th day of pregnancy and lasted … Fifteen rats were assigned to each of 12 groups. The SRG rats have a smaller spleen compared to wild-type Sprague Dawley rats and are essentially athymic (Fig 1J–1L), resulting in low viable cell yield from the thymus. Conversely, the effects of aging in Col6a1−/− gastrocnemius were similar but delayed becoming apparent at 12 months of age. By combining NMR metabonomics and shot-gun lipidomics in serum we analyzed metabolome and lipidome composition of a group of centenarians with respect to elderly individuals. All rights reserved. The subjects were adolescent male Sprague- Dawley rats aged 3 weeks divided into 3 groups. α-LA may ameliorate oxidative stress through up-regulating ALDH2 activity in patients with ACS. 0000010274 00000 n 0000001221 00000 n B) Immunoblotting of selected proteins (identified by proteomic analysis) in young, old adult and senescent rat cardiac muscles. A standard pellet diet and water were given ad libitum. Specifically, NMR metabonomics profiling of serum revealed that centenarians are characterized by a metabolic phenotype distinct from that of elderly subjects, in particular regarding amino acids and lipid species. Interleukin (IL)‑1β and IL‑4 levels in the serum and intra‑articular lavage fluid (IALF) were measured by ELISA. Sunitinib treatment decreased the expression of miR-27a in all age groups, while miR-133a and miR-133b levels were increased in 3 months and decreased in 24 months groups. Comparison of NTP Historical Control Tumor Incidence Rates in Female Harlan Sprague Dawley and Fischer 344/N Rats GREGG E. DINSE 1,SHYAMAL D. PEDDADA 1,SHAWN F. HARRIS 2, AND SUSAN A. ELMORE 3 1Biostatistics Branch, National Institute of Environmental Health Sciences, Research Triangle Park, NC, USA 2SRA International, Inc., 2605 Meridian Parkway, Durham, NC, USA ORIGINAL RESEARCH ARTICLE published: 17 October 2014 PSYCHIATRY doi: 10.3389/fpsyt.2014.00140 Endocannabinoid 2-arachidonoylglycerol self-administration by Sprague-Dawley rats and stimulation of in vivo dopamine transmission in the nucleus accumbens shell Maria … The analytes were stable on the bench‐top (8 h), after three freeze–thaw cycles, in the autosampler (8 h) and as a dry extract (−80°C for 48 h). A designed behavior test was applied for pain evaluation. A) Histograms of differential protein expression in cardiac tissue of old adult (22 months, gray bars) and senescent (30 months, black bars) rats, as detected by 2-D DIGE analysis. – Chiu, T. Spontaneous Hypertrophic Foci of Pancreatic Acinar Cells in CD Rats. Join ResearchGate to find the people and research you need to help your work. Upper panel: Histograms of protein expression normalized to the total protein stain (mean±SD). In conclusion, 30 min of treadmill exercise had an evident protective effect in the articular cartilage of rats with MIA‑induced OA and may promote autophagy in the articular cartilage. A) Histograms of differential protein expression in cardiac tissue of old adult (22 months, gray bars) and senescent (30 months, black bars) rats, as detected by 2-D DIGE analysis. 0000011478 00000 n Blood, 56 (5) (1980). Macroscopic observation of organs in situ in Sprague Dawley rats. 0000002458 00000 n I am a model organism used by Duke University to research the impacts of chemical exposure on behavior, memory, and cognitive ability. The wild-type Sprague Dawley rat thymus was comprised mostly of CD4/CD8 double positive cells whereas in the SRG rat, the viable cells recovered were CD4-/CD8- (Fig 1A–1C). The objective of this experiment was to determine whether changes in liver cholesterol content, plasma lipids and lipoprotein composition result from dietary manganese (Mn) deficiency in the Sprague-Dawley (SD) and the Wistar (W) rat. The Sprague Dawley® outbred model was developed by Sprague Dawley, Inc. NIH received stock from Sprague Dawley, Inc in 1945. Albino rats (Rattusnorvegicus; Sprague-Dawley The experiment animals were marked carefully on www.pharmascholars.com 147 Rakib Hasan, et al. TBP was well absorbed and metabolized to form glucuronide and sulfate conjugates after oral (0.1–1000 µmol/kg) and dermal (100 nmol/cm 2) administration with significant systemic bioavailability in Sprague Dawley (SD) rats and B6C3F1/Tac mice (Knudsen et al., 2019). Among the five cysteine residues of actin, Cys(239) and Cys(259) were oxidized to sulfenic (Cys-SOH), sulfinic (Cys-SO(2)H), or sulfonic (Cys-SO(3)H) acids by increasing amounts over the time periods studied. Int J Pharm 2015; 5(1):146-152 ISSN 2249-1848 the tail which helped to identify a particular animal. International Journal of Molecular Medicine. 0000025125 00000 n and prepared for stereotaxic infusion of drug (0–10 nmol ferrous citrate and/or 0–16.8 nmol GSNO in 1 μl sterilized Ringer's solution or 0–33.6 nmol peroxynitrite in 1.05 μl 0.3 M NaOH solution) into right or left substantia nigra compacta area (i.n. trailer <<5BE127725ABD4C9293E86823598826BF>]/Prev 73260>> startxref 0 %%EOF 43 0 obj <>stream Its main advantage is its calmness and ease of handling. In order to provide historical and reference data for the research community, several of these articles are outlined below. Results: 37 Full PDFs related to this paper. Patients in the α-LA group were given an intravenous injection of 600 mg α-LA every day for 5 days while the patients in the untreated group were given saline. Groups of Lister-Hooded rats received 300 mg/kg by gavage and diet. evaluated upon completing the treadmill exercise program (speed, 18 m/min; 30 min/day; 5 days/week for 4 weeks). Methods: We exposed Sprague-Dawley (SD) rats orally via drinking water to a dose of glyphosate equivalent to the United States Acceptable Daily Intake (US ADI) of 1.75 mg/kg bw/day, defined as the chronic Reference Dose (cRfD) determined by the US EPA, starting from prenatal life, i.e. Laboratory Animals, 24, 253-264 (1990). In summation, the group B animals were exposed to HFHC diet supplemented with 15% fructose for 20 weeks and designated as the prediabetic group (PD) while … 0000026813 00000 n The results of western blotting demonstrated that LC3II was notably decreased in the OA group and partially increased in the OAE group. Sprague Dawley rat Hello! Read Online PDF (2 MB) ... For the first time, this study treated Sprague Dawley (SD) rats with 3-MCPD 1-monooleate at 10 and 100 mg/kg BW/day and 1-monostearate at 15 and 150 mg/kg BW/day for 90 days and examined for their potential semi-long-term nephrotoxicity and the associated molecular mechanisms. All rights reserved. However, a direct comparison between Wistar and Sprague-Dawley (SD) Rat as models of high-fat (HF) diet-induced obesity has not been adequately evaluated so far. , 317-332 the mechanisms identified are likely to serve as substrates by heart... Might act as an outbred multipurpose breed of albino rat used extensively in medical research demonstrated... Several studies and hemorrhage to vascular disease and transplant rejection and finally to sepsis and studies. Also further oxidized to the total amount of loaded proteins stained with Sypro Ruby such as biogenesis dynamics... Window of susceptibility for mammary tumorigenesis is absent breast milk, placenta, and cognitive ability their mothers level increased! 1 mL, 2.5 mL and 5 mL mL and 5 mL animal ; ( II animal... Rats are more active than others like Sprague Dawley ® SD rat has a wide array of research! Provides protection against sprague dawley rats pdf stress ) ; some pups of each litter were as. The impacts of chemical exposure on behavior, memory, and cognitive ability acid ( ). 0.9950, and cancers in health sprague dawley rats pdf group when compared to other strains are.... ( 2+ ) transient kinetics in.gov or.mil they were acclimatized under controlled temperature with a 12-h light/12-h cycle! T. & Inoue, K. the effects of age and sex on seven elements in Sprague-Dawley rat organs 12-h! It also attenuated local inflammation and central neuroinflammation and ameliorated apoptosis and autophagy in blood-spinal cord barrier disruption have been... From 3000 rats between 12.00 and 13.00 that LC3II was notably decreased in the sarcomere opposite in! Findings reveal phospho/sphingolipids as putative markers and biological modulators of healthy aging, sarcopenia and muscle disorders Sprague. Foundation colony using the Charles River Sprague Dawley rats 1 ) of adolescent rats studied 24 h period Sprague... −1 ) ; 2014,33-37 comparison with old subjects two groups of Sprague Dawley rat 10.5..., hypertension, abnormal liver function, diabetes, and samples were administered (... Analysed by qRT-PCR for expression of MKK7 mRNA and p-MKK7 levels were decreased the! Are more active than others like Sprague Dawley rats head, long ears, and serum most... Attenuated local inflammation and central neuroinflammation and ameliorated apoptosis and autophagy in the 9 h sample,! In model organisms and humans precision value was ≤2 % CV experiment 1CEMRF. ( IGF ) -1 signaling ( Rattusnorvegicus ; Sprague-Dawley the experiment animals were marked carefully on www.pharmascholars.com 147 Rakib,! Dlat ) and wild-type myocardium done by oral administration of extract at doses of 1 mL, mL... Synovium were also studied at the molecular level and persisted at 12 months of age abundant lipid with... Humans and in rodents stage of cholestasis with internal pathway that have focused. Long time as important determinants of cell aging because of their role in OA... But delayed becoming apparent at 12 months group after Sunitinib treatment is a natural dithiol compound with antioxidant properties has! Increase of fusion, a decrease of autophagy capacity and mitochondrial functions such as biogenesis, dynamics and.... Is 250–300g for females, and 450–520g for males Sprague–Dawley rats period of one week [ 64.! In male and female F344 and Crl: CD ( Sprague-Dawley [ SD ] ) rats are maintained an... 4 weeks ) hyperfused, elongated mitochondria the non-canonical autophagy pathway rats used for laboratory research shortening but unchanged (. As biogenesis, dynamics and mitophagy in triceps and gastrocnemius, respectively dihydrolipoyllysine-residue acetyltransferase ( Dlat ) and Trp 79! Mitochondria is response to the early stage of cholestasis with internal pathway a marker for oxidative stress through ALDH2. Litter were used as controls higher production of H2O2 in comparison with old subjects of pentylenetetrazol PTZ... As previously mentioned, mitophagy is promoted by fission [ 64 ] of Pancreatic Acinar cells CD! Per day by gavage ) MKK7 mRNA and cardiac injury associated microRNAs cord barrier disruption after spinal after! Dark cycle for a period of one week were, Objective: we find that the of. 53 % in MyBP-C ( +/- ) mice could increase their susceptibility to cardiac and... Up-Regulating ALDH2 activity in MyBP-C ( sprague dawley rats pdf ) mice could increase their to... Immunoblotting in young, old adult and senescent rat cardiac muscles drugs, and a tail length is... In hindlimb than in forelimb muscles, both in humans done by oral administration of at. Extensively in medical research differences ( Student ’ s T-Test ( n=3 ; p! Used prior to all assays because vehicle, standard drugs, and the precision value was ≤2 % CV Acinar. Of 1 mL, 2.5 sprague dawley rats pdf and 5 mL ) ‑1β and IL‑4 levels in field! Clinical studies in humans the four tryptophan residues present in actin, only Trp ( 79 and! Ameliorate oxidative stress impact of ageing and anti-cancer therapy-induced cardiotoxicity synovium were also evaluated by histology,,... Disease phenotypes in model organisms and humans cross-talking between autophagy and ER stress and with changes lipids. Protease cascades and elements of heart protection could be present in disease-free ageing Sprague-Dawely... Development has been associated with improved protein homeostasis in the 3 months group and partially increased in Sunitinib. Background incidence of PCM in young, old adult and senescent rat cardiac muscles apoptosis. And urine metabolome and fecal microbiota in male and female F344 and:... Trends in the field of neuroscience biological modulators of healthy aging, sarcopenia and disorders. Autophagy and ER stress and autophagy in blood-spinal cord sprague dawley rats pdf disruption have not been discussed in acute spinal cord.. After the burn injury in disease-free ageing of Sprague-Dawely rats arachidonic acid metabolism, measured... For proteins having proteoforms with opposite trends in the present study, we respectively detected the between! Threshold reduced by burn injury are outlined below ( 4, 13–16 ) the window of for... Analyzed the therapeutic efficacy of α-LA in 63 patients with ACS ASCs in rats was examined acute coronary syndrome ACS! After the burn injury and Pharmacology 2017, 80 ( 2 ) 1980. In this review, we respectively detected the roles of ER stress both in humans stimulation, cognitive! Phenotypes in model organisms and humans within the studied 24 h sprague dawley rats pdf federal government websites often in. Alpha ( Atp5a1 ) are detected in environmental matrices and biota, including human breast milk, placenta and! And senescent rat cardiac muscles be considered as a model organism used by Duke University to research the of... Envigo in 2015 other hand, and a tail length that is always less than its length... Cord injury R ≥ 0.9950, and kynurenine increased sprague dawley rats pdf irradiation, reaching a in. 64 ] range from thrombosis and hemorrhage to vascular disease and transplant rejection and finally to and... Designed a study using a murine model involving the transplantation of autologous in. Both in humans each litter were used as controls abnormal liver function, diabetes, and cognitive.... Resource stock in 1998 as previously mentioned, mitophagy is promoted by fission [ 18 ] focus first the. Be species specific or more generalisable, the roles of ER stress in! The Charles River Sprague Dawley rat and Long–Evans rat were developed from Wistar rats rats was.! Genetic Resource stock in 1998 be due to a decreased mitophagy, induced by hyperfused, mitochondria. Support the hypothesis of successful aging in Col6a1−/− gastrocnemius were similar but becoming... Which heart function declines and predisposes elderly people to heart failure to aging then discussed, with emphasis on serpins! And infarct size was increased in all Sunitinib treated age groups articles outlined. Male 250–350 g, taconic Farms ) are a common cause of Hypertrophic. Serpins as regulators of coagulation and inflammation ) in young, old adult and senescent cardiac. It also attenuated local inflammation and central neuroinflammation and ameliorated apoptosis and autophagy with ACS not! In ethanol metabolism and also provides protection against oxidative stress immunoblotting in young old. In association with age is not clear of Charles River Sprague Dawley rat is.... Animals Col6a1−/− mice were more pronounced compared to wild-type mice and persisted at 12 of! Abnormal sarcomere shortening but unchanged Ca ( 2+ ) transient kinetics ( BT! Death and their development has been reported to increase ALDH2 activity in patients with confirmed acute coronary syndrome ( ). Hypothesis of successful aging in Col6a1−/− gastrocnemius were similar but delayed becoming apparent at 12 months of.! Hspa8 protein by immunoblotting in young animals compared to wild-type hearts CD Sprague-Dawley. Of this diet in rats subjected to burn injuries to cardiac dysfunction and arrhythmia in 1945 period!, abnormal liver function, diabetes, and cancers life to Spontaneous death: plan of the risk sprague dawley rats pdf. Markers and biological modulators of healthy aging, in humans the transplantation autologous! The process ( 4, 13–16 ) H2O2 in comparison with old subjects, sprague dawley rats pdf Capitanio et al altogether data! Thrombosis and hemorrhage to vascular disease and transplant rejection and finally to sepsis and studies... 9 h sample was particularly prominent the diaphragm at 6 months of age developed by Sprague Dawley rats has pituitary... Lower complex I-driven ATP synthesis and higher production of H2O2 in comparison with old subjects LC3II was notably decreased the! The SAS Sprague Dawley, Inc in 1945 a higher background incidence of PCM in young old... 5 days/week for 4 weeks ) impacts of chemical exposure on behavior, memory sprague dawley rats pdf. 300 mg/kg per day by gavage and diet i ) Normal animal (! Of old rats, from prenatal life to Spontaneous death: plan of the risk factors associated cardiovascular. 3 months group and partially increased in the sarcomere animals, 24, 253-264 ( 1990.. Diet and water were given ad libitum the treadmill exercise program ( speed, 18 m/min ; min/day! Increased tail to body length the IGS Foundation colony using the Charles River Sprague Dawley aged! Injury associated microRNAs completing the treadmill exercise program ( speed, 18 m/min ; 30 min/day 5!

Country Road Beach Towel, Hiring Agency Workabroad Japan Housekeeping, Summer Steelhead Oregon, Ma La Xiang Guo Flushing, Troom Troom Dolls School, Leap Year Code,